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Biopharmaceutical Analysis Research Group


Dr Karnes and Dr Halquist combine over 45 years of biopharmceutical analysis research experience.  The Biopharmaceutical Analysis Research Group investigates existing and new tools for answering quantitative questions. We also collaborate with many investigators within the scientific community to address a variety of biologic and non-biologic issues and in this way contribute to the overall progress of science.

Biorelevant In Vitro Release Testing

Design and builder in vitro devices that can be validated for accurately predicting in vivo bioavailability. This approach provides a better estimate of exposure or therapeutic potential from a variety of vehicles and aids in the selection of optimal conditions for further study.

Capillary System Using Bioreactor to Study Drug Release of Naltrexone Implant

Pubmed link to abstract: http://www.ncbi.nlm.nih.gov/pubmed/17482393

Bidirectional Apparatus for Transmucosal Dosage Form Nicotine Release from General Snus

Pubmed link to abstract: http://www.ncbi.nlm.nih.gov/pubmed/22486954

 

Protein Quantification Mass Spectrometry

Quantitative analysis of peptides and proteins using the signature peptide approach and/or multiply charge intact peptides. The signature peptide approach offers a unique targeted peptide as the intact protein surrogate for quantification as a more selective alternative to traditional ligand binding assays. We have also quantified smaller proteins and peptides using mass spectrometry without the need for proteolytic digestion by selected reaction monitoring (SRM) of the multiply charged species [MN+]. 

Links to current Pubmed papers about these approaches [To Be Added]

Dosage Form Characterization

We have evaluated various dosage forms (i.e. suspensions) for potency, purity, stability, forced degradation studies, and stability indicating method development and validations

Bioanalysis

Bioanalytical LaboratoryFor over thirty years, our Bioanalytical laboratory has been collaborating with the scientific community as a shared resource and service center to answer quantitative questions related to biologic and non-biologic bio/pharmaceutical research.

Small and large molecule quantification (HPLC-UV/PDA/Fluor/ and LC-MS/MS) in biological fluids